Femoropopliteal and femorotibial bypass procedures on patients with diabetes mellitus: Medium term follow-up results

Koksal C, Besirli K, Bozkurt AK, Kaynak MK, Cangel U, Sayin A

Cerrahpasa J Med 1999; 30: 203-206.

BACKGROUND AND DESIGN: Thirty-five patients with diabetes mellitus underwent 30 femoropopliteal and 5 femorotibial bypass procedure. This group was matched with 35 cohort of patients without diabetes mellitus which were selected from the vascular database. RESULTS: Cumulative patency in diabetics for 1 and 3 years were 88.6% and 77.1% respectively. The results in non-diabetics were 91.4% and 84.4%, respectively (p>0.05). Six minor and 3 major amputations had to be performed on diabetics contrary to the 2 minor and 1 major amputations in non-diabetics (p<0.05). CONCLUSION: Results achieved in diabetic group were not statistically significant than the non-diabetic group although amputation rate was found higher in the former. (Return)

Effect of iron overloading on the tissue levels of iron

Seymen HO, Ozcelik D, Gulyasar T, Mengi M, Seymen P, Yigit G

Cerrahpasa J Med 1999; 30: 207-213.

BACKGROUND AND DESIGN: The harmful effects of acute and chronic iron loading are well documented. Increased lipid peroxidation is an important expression of both chronic and acute iron toxicity. Our aim was to study the effect of iron overloading on the different tissue levels of iron. RESULTS: Fourteen adult male Wistar albino rat weighing 200-250 g were used. The animals were divided into 2 weight-matched groups. Group 1: Control group (n=7) and Group 2: Iron overloading group (n=7). The rats in group 2 received intraperitoneal iron (Ferro III hydroxide polymaltose) at a dose of 250 mg/kg/day for 10 days. At the end of the administration period, the rats were anesthetized by ketamine (50 mg/kg). Heparinized blood samples were obtained via aorta abdominalis. The samples of the tissue from liver, heart, spleen, brain and skeletal muscle were obtained. The plasma and the tissue levels of iron were measured by atomic absorption spectrophotometer. The plasma free iron values for rats receiving iron were significantly (p<0.001) higher. This finding confirmed the establishment of the iron overloading state. The liver, heart, spleen, and skeletal muscle tissue levels of iron were significantly higher (p<0.001, p<0.01, p<0.05, p<0.05 respectively) than those of control rats. There was no significant difference between two groups brain tissue levels of iron (p>0.05). CONCLUSION: Our results show that the liver cells have been the most capability of iron storage. (Return)

Ventilatory-associated pneumonia

Meyanci G, Oz H, Mamal Torun M

Cerrahpasa J Med 1999; 30: 214-220.

BACKGROUND AND DESIGN: We aimed to point out the pneumonia incidence in mechanically ventilated patients to define the prognosis and mortality rate in patients diagnosed to have pneumonia in Intensive Care Unit (ICU). In our study we reviewed 55 patients who were ventilated for at least 3 days and 28 patients out of 55, diagnosed to have pneumonia are included the study. We verified pneumonia diagnosis according to infiltrations on chest X-ray and accompanying at least two clinical signs. For each patient studied, the chart is reviewed for the following parameters: ECG, invasive arterial pressure, central venous pressure, body temperature, microbiological analyses of tracheal aspirate, whole body count, electrolyte, hemocultures, urine culture and pneumonic infiltration shown on chest X-ray. The antibiotic therapy schedule was devised as double or triple therapy according to antibiogram. RESULTS: Pneumonia incidence is found to be 50.6% and mortality incidence 61%. According to microbiological analyses of tracheal aspirates, S. aureus encountered in 7 cases, P. aeuroginosa in 5 cases, enterococcus'spp in 4 cases, acinetobacter'spp in 2 cases, stenotrophomonas maltophilia in one case and H. influenzae in one case. In 7 cases polymicrobial pneumonia was the case. CONCLUSION: Nosocomial pneumonia caused by polymicrobial and multiresistant microorganisms, appears as a disease with high mortality rate.(Return)

Capillary gel electrophoresis of PCR products of HLA DQA1

Yilmaz S, Cengiz S

Cerrahpasa J Med 1999; 30: 221-227.

BACKGROUND: HLA DQA1 loci are highly informative polymorphic loci that are gaining popularity for identity testing. HLA DQA1 system easy to work with, fast and reliable. This loci with PCR technique is a very promising tool for genetic investigations in both paternity and crime cases. Design.- Human genomic DNA was extracted by Chelex 100 procedure. The amplification of HLA DQA1 locus was performed by single locus PCR reaction in the case of HLA DQA1 locus according to the manufacturer's recommendations using the Perkin Elmer AmpliType DQA1 PCR Amplification Typing Kits. The electrophoresis of PCR products of HLA DQA1 are carried out on agarose gel. RESULTS: It was shown from the obtained bands that the DNA is amplified. Hybridization is made from the PCR products by Perkin Elmer AmpliType DQA1 PCR Amplification Typing Kits and that typed as 2,3 as a result and this allele was compared with the Capillary electrophoreograms. CONCLUSION: In this study, Capillary gel electrophoresis (CGE) was performed on PCR products of HLA DQA1 due to its advantages are ultra-resolution, ultralow sample volume, extremely high efficiency, rapid separation time, easy quantitation and amenability to automation.(Return)

A case of Castleman's disease who's HBsAg carrier resorting with hepatosplenomegaly

Buyukbese MA, Ozdemir S, Aki H, Soysal T, Pekmezci S, Basaranoglu M, Ongoren S, Sonsuz A, Akin P

Cerrahpasa J Med 1999; 30: 228-232.

OBSERVATION: Twenty-six year old male patient admitted to outpatient clinics of Hepatology Department because of the complaints of hiccups, malaise, and decreased exercise tolerance. HbsAg had been positive for about eleven years. Physical examination revealed hepatosplenomegaly and a suspected mass at the right inferior quadrant of the abdomen. In the computed tomography in addition to hepatosplenomegaly, a mass of 70x60x40 mm in dimension was noticed at the root of the mesentery and a decision for laparotomy was made. The histopathology of the mass extracted was documented as Castleman's disease of plasma cell variant. Two years later, he was doing well. Physical examinations were unremarkable, and complete blood counts, biochemical and radiologic examinations were within normal limits. Literature is reviewed because of this case. (Return)

Acute epidural hematoma: A Case report

Bozkus H, Uzan M, Oguz E, Ciplak N, Kuday C

Cerrahpasa J Med 1999; 30: 233-235.

BACKGROUND AND OBSERVATION: There are several reports regarding the rapid resolution of acute epidural hematoma. There are some possible mechanisms may involve in these cases. A 2-year-old girl fell from a height and Children Coma Score was 15/15. CT scan revealed a linear fracture on the temporal bone and a right side temporoparietal acute epidural hematoma. The patient was observed in the clinic and control CT scan revealed disappearance of hematoma in the posttraumatic 12th hour. Evidence of a fracture in the rare cases of acute epidural hematoma can lead rapid resolution of epidural hematoma with an increase in the size of scalp hematoma. (Return)

Some important changes in taxonomy of Candida albicans

Yucel A, Kantarcioglu AS

Cerrahpasa J Med 1999; 30: 236-246.

BACKGROUND: The genus Candida is classified in the family Cryptococcaceae and included about 200 species in recent taxonomic treatise. C.albicans is the more frequent pathogenic species and is also part of normal human flora. Recently, germ tube and chlamydospore positive Candida albicans strains with abnormal characteristics have been isolated from primarily oral candidiasis in HIV infected (HIV+) individuals and AIDS patients. This isolates have very close phenothypic properties to those of C.albicans but a distinctive atypical genomic organisation. First identified as a new species of Candida in Ireland in 1995 and the name Candida dubliniensis has been proposed. C.stellatoidea, C.claussenii and C.langeronii has been reduced to synonymy of C.albicans on the basis of their significant DNA relatedness. (Return)